Ornithine Decarboxylase-1 (ODC-1) Mouse Monoclonal Antibody [Clone ID: ODC1/485]

CAT#: AM50303PU-T

Ornithine Decarboxylase (ODC1) mouse monoclonal antibody, clone ODC1/485, Purified

Product Images

Western blot analysis of ODC1 in human placental lysate using ODC-1 Antibody (Clone ODC1/485).

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  Formalin-fixed, paraffin-embedded human prostate carcinoma stained with ODC-1 Antibody (Clone ODC1/485)

Specifications

Product Data

• Clone Name: ODC1/485
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Flow Cytometry: 0.5-1 μg/million cells.
  Immunofluorescence: 0.5-1 μg/ml.
  Western Blotting: 0.5-1 μg/ml.
  Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1.0 μg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Recommended Positive Control: Epithelial cells in normal placenta or prostate carcinoma.
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant full-length human ODC-1 protein
• Specificity: Cellular Localization: Cytoplasm, Cytosol
• Formulation: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 53kDa.
• Gene Name: ornithine decarboxylase 1
• Database Link:
  Entrez Gene 4953 Human
  UniProt ID P11926
• Background: Recognizes a 53kDa protein, identified as the Ornithine Decarboxylase (ODC-1). ODC is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. ODC activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.
• Synonyms: Ornithine decarboxylase structural 1; RNODC