14 3 3 gamma (YWHAG) Mouse Monoclonal Antibody [Clone ID: AT4B9]
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Specifications
Product Data | |
Clone Name | AT4B9 |
Applications | ELISA, IF, WB |
Recommended Dilution | ELISA. WB: 1/1000 (using lysates of HeLa (40µg) and mouse brain (40 µg), resolved by SDS-PAGE and transferred to PVDF membrane) ICC/IF: 1/100 (using HeLa cell line). |
Reactivities | Human |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Recombinant human 14-3-3 gamma (1-247aa) purified from E. coli. |
Specificity | Recognizes Human 14-3-3 gamma (YWHAG). Other species not tested. |
Formulation | PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol State: Purified State: Liquid purified Ig fraction |
Concentration | lot specific |
Purification | Protein-A affinity chromatography |
Storage | Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Gene Name | Homo sapiens tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma (YWHAG) |
Database Link | |
Background | The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, Beta, Gamma, Epsilon, Delta, Zeta, Tau and Eta that have been identified in mammals. The 14-3-3 gamma, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. |
Synonyms | YWHAG, KCIP-1 |
Reference Data | |
Protein Families | Druggable Genome |
Protein Pathways | Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis |
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