PMVK Mouse Monoclonal Antibody [Clone ID: AT21C10]

CAT#: AM50615PU-S

PMVK mouse monoclonal antibody, clone AT21C10, Purified

Product Images

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PMVK antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : HepG2 cell lysate Lane 3. : Jukat cell lysate Lane 4. : MCF7 cell lysate Lane 5. : A549 cell lysate

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  ICC/IF analysis of PMVK in PC3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PMVK antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Specifications

Product Data

• Clone Name: AT21C10
• Applications: ELISA, FC, IF, WB
• Recommended Dilution: The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
• Reactivities: Human
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Recombinant human PMVK (1-192aa) purified from E. coli.
• Formulation: Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Protein-A affinity chromatography
• Gene Name: Homo sapiens phosphomevalonate kinase (PMVK), transcript variant 1
• Database Link:
  Entrez Gene 10654 Human
• Background: Phosphomevalote kise, also known as PMVK, is a cytosolic enzyme. It was expressed highly in heart, liver, skeletal muscle, kidney and pancreas and slightly lower in brain, placenta, and lung. Induced by sterol, PMVK participates in isopentenyl diphosphate biosynthesis via the mevalote pathway. PMVK catalyzes the conversion of mevalote 5-phosphate into mevalote 5-diphosphate in the fifth reaction of the cholesterol biosynthetic pathway.
• Synonyms: Phosphomevalonate kinase, PMKase, PMKI, HUMPMKI

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Terpenoid backbone biosynthesis