Fumarase Mouse Monoclonal Antibody [Clone ID: AT3F6]

CAT#: AM50626PU-N

Fumarase mouse monoclonal antibody, clone AT3F6, Purified

Product Images

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Fumarase antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : LNCap cell lysate Lane 3. : A549 cell lysate Lane 4. : HepG2 cell lysate Lane 5. : 293T cell lysate Lane 6. : K562 cell lysate Lane 7. : Jurkat cell lysate

Specifications

Product Data

• Clone Name: AT3F6
• Applications: ELISA, WB
• Recommended Dilution: The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant human Fumarase (44-510aa) purified from E. coli.
• Formulation: Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Protein-A affinity chromatography
• Gene Name: Homo sapiens fumarate hydratase (FH)
• Database Link:
  Entrez Gene 2271 Human
• Background: Fumarase (Fumarate hytdratase) is an enzyme that catalyzes the reversible hydration/dehydration of fumarate to S-malate and is involved in the tricarboxylic acid (TCA), or Krebs cycle. This enzyme exists in both a cytosolic form and an N-termil extended mitochondrial form. The N-termil extended form is targeted to the mitochondrion, where the removal of the extension is the same form as in the cytoplasm. Fumarase deficiency can lead to progressive encephalopathy, cerebral atrophy and developmental delay and this enzyme also is thought to act as a tumor suppressor.
• Synonyms: HLRCC, LRCC, MCL, MCUL1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Citrate cycle (TCA cycle), Metabolic pathways, Pathways in cancer, Renal cell carcinoma