Caspase 3 (CASP3) (active) Rabbit Polyclonal Antibody

CAT#: AP00003PU-N

Caspase 3 (CASP3) (active) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of caspase-3 in camptothecin (2 uM) treated Jurkat cells.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/50-1/500.
  Immunohistochemistry on Paraffin Sections: 1/10-1/20.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide mapping to the N-terminus adjacent to the cleavage site of Human Caspase-3.
• Specificity: The antibody preferentially recognizes the p17 fragment of the active Caspase-3.
• Formulation: PBS containing 30% Glycerol, 0.5% BSA, and 0.01% Thimerosal
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Antigen Affinity Chromatography
• Storage: Upon receipt, store (in aliquots) at -20°C to -80°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 836 Human
• Background: Caspase-3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP). The affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
• Synonyms: CASP-3, CASP3, CPP32, CPP-32, Yama protein, Apopain, SCA-1, SCA1