Caspase 1 (CASP1) Rabbit Polyclonal Antibody

CAT#: AP00006PU-N

Caspase 1 (CASP1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Caspase-1 expression in Rat liver tissue lysate using Caspase-1 Antibody

Specifications

Product Data

• Applications: IF, IP, WB
• Recommended Dilution: Western Blot: 0.5-4 µg/ml.
  Immunoprecipitation: 4-8 µg/ml.
  Immunofluorescence: 10-20 µg/ml.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide surrounding amino acid 132 of Human Caspase-1.
• Specificity: This antibody recognizes 45 kDa Procaspase-1 and the 20 kDa cleaved product.
• Formulation: PBS, pH 7.2, 0.5mM EDTA
  State: Aff - Purified
  State: Liquid purified IgG fraction
  Stabilizer: 0.5% BSA, 30% Glycerol
  Preservative: 0.03% Proclin^®
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Storage: Upon receipt, store the antibody undiluted at -20°C or for long term storage (in aliquots) at -70°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 834 Human
• Background: Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase 1 is similar to the cell death gene CED3 of C. elegans and regulates multiple proinflammatory cytokines, including Interleukin 1b and interferon-gamma-inducing factor. Caspase 1 plays a role down stream of Caspase 8 which is involved in Fas-mediated apoptosis.
• Synonyms: CASP-1, CASP1, IL1BC, IL1BCE, ICE, p45
• Note: Blocking peptide (Cat.-No AP00006CP-N) is available separately.