Caspase 8 (CASP8) (active) Rabbit Polyclonal Antibody

CAT#: AP00055PU-N

Caspase 8 (CASP8) (active) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of Caspase-8. Lane 1: Recombinant h-caspase-8; Lane 2: Untreated Jurkat cells; Lane 3: Camptothecin induced cells.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 0.5-4 μg/ml.
  Immunohistochemistry: 10-20 mg/ml.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide surrounding the cleavage site of human
  Caspase-8
• Specificity: The affinity purified antibody mainly detects the 26 kDa fully cleaved fragment and the 35-43 kDa intermediate of caspase-8 in apoptotic samples.
• Formulation: PBS, pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal
  State: Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Affinity chromatography
• Storage: Store the antibody undiluted at -20°C or for long term storage (in aliquots) at -70°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 841 Human
• Background: Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-8 is a 55 kDa cytosolic protein that is synthesized as an inactive pro-enzyme. Activation of caspase-8 involves a two-step proteolysis: the cleavage of caspase-8 to generate a 43 and a 12 kDa fragment which is further processed to 10 kDa. The p43 is then cleaved to yield p26 and the release of the active site containing p18.
• Synonyms: CASP-8, CASP8, MCH5, CAP4