HRK Rabbit Polyclonal Antibody

CAT#: AP00058PU-N

HRK rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western Blot: 1 μg/ml.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to residues surrounding amino acids 50 of human HRK.
• Specificity: The antibody detects Hrk fused with green fluorescent protein in crude extracts of E. coli transfected with Hrk-GFP.
• Formulation: PBS, pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide
  State: Aff - Purified
  State: Liquid purified Ig
• Concentration: 0.5 mg/ml
• Purification: Affinity purified
• Gene Name: Homo sapiens harakiri, BCL2 interacting protein (HRK), transcript variant 1
• Database Link:
  Entrez Gene 8739 Human
• Background: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Hrk, a pro-apoptotic member of the Bcl-2 homology domain-3 (BH3) group of the Bcl-2 family of proteins, was identified as a novel protein induced during programmed neuronal death. It lacks significant homology to other Bcl-2 family members except for an 8-amino acid region that is similar to the BH3 motif of Bik. Hrk regulates apoptosis through interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL via this domain. It does not interact with the pro-apoptotic proteins Bax, Bak, or Bcl-XS. Hrk localizes to mitochondrial membranes in a pattern similar to that previously reported for Bcl-2 and Bcl-XL. Despite its predicted molecular weight, Hrk often migrates at 12-15 kDa.
• Synonyms: Neuronal death protein DP5, BID3

Reference Data

• Protein Families: Druggable Genome, Transmembrane