Cathepsin B (CTSB) Rabbit Polyclonal Antibody

CAT#: AP00064PU-N

Cathepsin B (CTSB) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of Cathepsin B with Jurkat cell (Lane 1) and mouse small intestine tissue (Lane 2) lysate.

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  Flow Cytometry analysis of Cathepsin B levels within phagosomes of macrophages

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: Western Blot: 0.5-4 µg/ml.
  Positive Control: Jurkat cell lysate & mouse small intestine tissue lysate.
  Immunohistochemistry: 1/100.
  Flow Cytometry: 1/50 (v/v).
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide surrounding amino acid 322 of Human Cathepsins B
• Specificity: The purified antibody detects both the proform (37-43 kDa) and the mature form (25 kDa) of Cathepsin B.
  It reacts with Human and Mouse and in a lesser extent with Rat samples.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified Ig fraction
  Stabilizer: 30% Glycerol, 0.5% BSA
  Preservative: 0.01% Thimerosal
• Concentration: lot specific
• Purification: Affinity Chromatography
• Storage: Store undiluted at -20°C or for long term storage (in aliquots) at -20°C to -70°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 1508 Human
• Background: Cathepsin B is a normal lysosomal cysteine proteinase that is expressed in all cells. It is synthesized as an inactive 43 kDa pro-enzyme which, by removal of a 62 amino acid propeptide, is activated to the single-chain form of 31 kDa or the two-chain form (25 and 5 kDa subunits). Matured cathepsin B is normally localized in the lysosomes where it functions in protein turnover. Cathepsin B is also shown capable of degrading extracellular matrix proteins. Elevated levels of cathepsin B correlate with malignancy suggesting that this enzyme may be a useful prognostic marker for several types of human cancers.
• Synonyms: Cathepsin B1, APP secretase, APPS, CTSB, CPSB