Caspase 9 (CASP9) Rabbit Polyclonal Antibody

CAT#: AP00153PU-N

Caspase 9 (CASP9) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Caspase-9 expression with mouse small intestine tissue lysate.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western Blot: 0.5-4.0 µg/ml.
  Mouse small intestine and rat kidney tissue lysates can be used as Positive Controls.
• Reactivities: Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide surrounding amino acid 345 of Mouse Caspase-9
• Specificity: The antibody detects 45 kDa procaspase-9, 35/37 kDa cleaved large fragment and the 25 kDa fully cleaved Caspase-9.
• Formulation: PBS, pH 7.2, containing 30% Glycerol, 0.5% BSA and 0.01% Thimerosal.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein A Chromatography.
• Storage: Store the antibody undiluted at -20°C or (in aliquots) at -70°C for long term storage.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens caspase 9 (CASP9), transcript variant alpha
• Database Link:
  Entrez Gene 12371 MouseEntrez Gene 58918 Rat
• Background: Caspase-9 is one of the most important caspases among the caspase family members. Upon induction of apoptosis, cytochrome c released from mitochondria associates with procaspase- 9/Apaf-1. The complex processes procaspase-9 into a large subunit (35 kDa or 17 kDa) and small (10 kDa) by self-cleavage at D315. Activated caspase-9 further cleaves other caspase members including caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. In addition to self-cleavage, procaspase-9 can also be cleaved in vivo by caspase-3 at D330. The process served as a positive feedback to amplify the apoptotic signal in caspase activation pathway.
• Synonyms: CASP-9, CASP9, MCH6, ICE-LAP6, APAF-3, APAF3

Reference Data

• Protein Families: Druggable Genome, Protease, Stem cell - Pluripotency
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Endometrial cancer, Huntington's disease, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Parkinson's disease, Pathways in cancer, Prostate cancer, Small cell lung cancer, VEGF signaling pathway, Viral myocarditis