DDIT3 Rabbit Polyclonal Antibody

CAT#: AP00161PU-N

DDIT3 rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: IHC, IP, WB
• Recommended Dilution: Western blot (0.5-4 μg/ml).
  Immunoprecipitation (10-20 μg/ml).
  Immunohistochemistry on paraffin sections (10-20 μg/ml).
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide surrounding amino acid 124 of human GADD153
• Specificity: The antibody recognizes 25 kDa GADD135.
• Formulation: PBS containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein A affinity chromatography
• Storage: Store at -20°C for up to one year from despatch. For longer storage, freeze at -70 °C. Avoid repeated freeze/defrost cycles.
• Database Link:
  Entrez Gene 1649 Human
• Background: GADD153 has been described as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. Expression of GADD153 is induced by a variety of cellular stresses, including nutrient deprivation and metabolic perturbations. GADD153 functions to block cells in G1 to S phase in cell cycle progression and acts by dimerizing with other C/EBP proteins to direct GADD153 dimers away from “classical” C/EBP binding sites. Thus GADD153 acts as a negative modulator of C/EBP-like proteins in certain terminally differentiated cells.
• Synonyms: DDIT3, DDIT-3, CHOP10, CHOP-10
• Note: Blocking peptide (AP00161CP-N) is also available separately.