Nsf Rabbit Polyclonal Antibody

CAT#: AP00234PU-N

Nsf rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: IHC, IP, WB
• Recommended Dilution: Western Blot: 1-2 μg/ml.
  Immunoprecipitation: 5-15 mg/ml.
  Immunohistochemistry: 10-20 μg/ml.
• Reactivities: Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to amino acids around 731 of rat NSF
• Specificity: The antibody recognizes 85 kDa NSF.
• Formulation: PBS containing 50 % glycerol, 1 % BSA, and 0.02 % sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 0.5 mg/ml
• Purification: Protein A chromatography
• Gene Name: Rattus norvegicus N-ethylmaleimide sensitive factor, vesicle fusing ATPase (Nsf)
• Database Link:
  Entrez Gene 18195 MouseEntrez Gene 60355 Rat
• Background: Syntaxins were originally thought to be docking proteins, but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins have been shown to bind to various proteins involved in exocytosis, including VAMPs (vesicle-associated membrane proteins), NSF (N-ethylmaleimidesensitive factor), SNAP 25 (synaptosomal-associated protein of 25kDa), SNAPs (soluble NSF attachment proteins) and synaptotagmin. SNAPs mediate the membrane binding of NSF, which is essential for membrane fusion reactions. An additional protein designated synaptophysin may regulate exocytosis by competing with SNAP 25 and syntaxins for VAMP binding.
• Synonyms: SKD2