MAPK8 / MAPK9 pThr183/pTyr185 Rabbit Polyclonal Antibody

CAT#: AP00260PU-N

MAPK8 / MAPK9 pThr183/pTyr185 rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: Western Blot: 1-2 µg/ml.
  Immunoprecipitation: 10-20 µg/ml.
  Immunocytochemistry: 10-20 µg/ml.
  Immunohistochemistry: 20-30 µg/ml.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr183/Tyr185 of Human SAPK/JNK
• Specificity: The antibody detects the p46 and p54 JNK/SAPK dually phosphorylated at Thr183 and Tyr185.
• Formulation: PBS, pH 7.2, containing 50% Glycerol, 1% BSA and 0.02% Sodium Azide
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: 0.5 mg/ml
• Purification: Affinity Chromatography.
• Background: The JNK pathway is activated by largely distinct stimuli including inflammatory cytokines (such as TNF-alpha and IL-1), UV light, inhibitors of protein synthesis and osmotic stress. Activated MEKK1 phosphorylates SEK1 (also known as MKK4), which in turn activates SAPK (also known as JNK). JNK binds tightly to the N-terminal region of c-Jun and ATF-2, and phosphorylates c-Jun at Ser63 and Ser73 and ATF-2 at Thr69 and Thr71.
• Synonyms: MAPK8, JNK1, PRKM8, SAPK, JNK-1, MAPK-8, JNK-46, MAPK9, MAPK-9, JNK2, PRKM9, JNK-2, JNK-55