JNK1 (MAPK8) Rabbit Polyclonal Antibody

CAT#: AP00339PU-N

JNK1 (MAPK8) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of JNK expression in Jurkat cell lysates (Lane 1, 2).

Specifications

Product Data

• Applications: IHC, IP, WB
• Recommended Dilution: Western Blot: 1 µg/ml.
  Immunoprecipitation: 10-20 µg/ml.
  Immunohistochemistry: 10-20 µg/ml.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide mapping to the C-terminus of human JNK-1
• Specificity: The antibody detects the 46 kDa JNK1.
• Formulation: PBS pH 7.2, containing 50% Glycerol, 1% BSA, 0.02% Thimerosal.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography.
• Storage: Store the antibody undiluted at -20°C or for long term storage (in aliquots) at -70°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 5599 Human
• Background: The JNK pathway is activated by largely distinct stimuli including inflammatory cytokines (such as TNF-alpha and IL-1), UV light, inhibitors of protein synthesis and osmotic stress. Activated MEKK1 phosphorylates SEK1 (also known as MKK4), which in turn activates SAPK (also known as JNK). JNK binds tightly to the N-terminal region of c-Jun and ATF-2, and phosphorylates c-Jun at Ser63 and Ser73 and ATF-2 at Thr69 and Thr71.
• Synonyms: Mitogen-activated protein kinase 8, c-Jun N-terminal kinase 1, JNK-46, JNK-1, PRKM8