WNT3A Rabbit Polyclonal Antibody

CAT#: AP00591BT-N

WNT3A rabbit polyclonal antibody, Biotin


USD 355.00

2 Weeks*

Size
    • 50 ug

Product Images

Specifications

Product Data
Applications ELISA, WB
Recommended Dilution Direct ELISA: To detect Human WNT-3a by Direct ELISA (using 100 μl/well antibody solution) a concentration of ~1.0 μg/ml is required. This biotinylated polyclonal antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Human WNT-3a.
Sandwich ELISA: To detect Human WNT-3a by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human WNT-3a (Cat.-No AP00591PU-N or AP00591PU-S) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human WNT-3a.
Western Blot: To detect Human WNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivities Human
Host Rabbit
Clonality Polyclonal
Immunogen E.Coli derived recombinant Human WNT-3a
Specificity This antibody detects Human WNT-3a. Other species not tested.
Formulation PBS, pH 7.2
Label: Biotin
State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification Affinity Chromatography
Conjugation Biotin
Synonyms Protein Wnt-3a
Note Centrifuge vial prior to opening.
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.