WNT3A Rabbit Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA, WB |
Recommended Dilution | Direct ELISA: To detect Human WNT-3a by Direct ELISA (using 100 μl/well antibody solution) a concentration of ~1.0 μg/ml is required. This biotinylated polyclonal antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Human WNT-3a. Sandwich ELISA: To detect Human WNT-3a by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human WNT-3a (Cat.-No AP00591PU-N or AP00591PU-S) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human WNT-3a. Western Blot: To detect Human WNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Reactivities | Human |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | E.Coli derived recombinant Human WNT-3a |
Specificity | This antibody detects Human WNT-3a. Other species not tested. |
Formulation | PBS, pH 7.2 Label: Biotin State: Lyophilized (sterile filtered) purified Ig fraction |
Reconstitution Method | Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. |
Purification | Affinity Chromatography |
Conjugation | Biotin |
Database Link | |
Synonyms | Protein Wnt-3a |
Note | Centrifuge vial prior to opening. |
Reference Data |
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