WNT3A Rabbit Polyclonal Antibody

CAT#: AP00591PU-S

WNT3A rabbit polyclonal antibody, Aff - Purified

Product Images

This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.125 mg/ml-0.25 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Sandwich ELISA: To detect Human WNT-3a by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml is required. This affinity purified polyclonal antibody, in conjunction with Biotin conjugated Polyclonal Anti-Human WNT-3a (AP00591BT-N or AP00591BT-S) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human WNT-3a.
  Western Blot: To detect Human WNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
  Immunohistochemistry: 0.125-0.25 µg/ml with an overnight incubation at 4°C.
  This antibody stained Formalin-Fixed, Paraffin-Embedded sections of Human breast invasive ductal carcinoma. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommneded. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: E.Coli derived recombinant Human WNT-3a
• Specificity: This antibody detects Human WNT-3a. Other species not tested.
• Formulation: PBS, pH 7.2 without preservatives
  State: Aff - Purified
  State: Lyophilized (sterile filtered) purified Ig fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography
• Database Link:
  Entrez Gene 89780 Human
• Synonyms: Protein Wnt-3a
• Note: Centrifuge vial prior to opening.