MCP4 (CCL13) Goat Polyclonal Antibody

CAT#: AP01117BT-S

MCP4 (CCL13) goat polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA: Direct: To detect hMCP-4 by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 - 1.0 μg/ml is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-4.
  Sandwich: To detect hMCP-4 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 - 1.0 μg/ml is required. In conjunction with Polyclonal Anti-Human MCP-4 as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-4.
  Western Blot: To detect hMCP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) recombinant human MCP-4
• Specificity: This antibody detects MCP-4.
• Formulation: PBS, pH 7.2
  Label: Biotin
  State: Sterile filtered lyophilized Ig fraction
• Reconstitution Method: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/ml.
• Purification: Affinity chromatography
• Conjugation: Biotin
• Storage: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at
  2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C. Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  Entrez Gene 6357 Human
• Background: Human macrophage/monocyte chemotactic protein 4 (MCP4) is a member of the b-chemokine family (C-C) of Cytokines. Like Eotaxin, MCP4 is a powerful eosinophil chemo-attractant. Additionally, like MCP3, MCP4 elicits chemo-attraction of monocytes and T lymphocytes.
• Synonyms: C-C motif chemokine 13, CCL13, MCP4, NCC1, SCYA13, CK-beta-10, NCC-1, Monocyte chemotactic protein 4