Eotaxin 2 (CCL24) Goat Polyclonal Antibody

CAT#: AP01125BT-S

Eotaxin 2 (CCL24) goat polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA: Direct: To detect Eotaxin-2 by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 - 1.0 μg/ml is required. In conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Eotaxin-2.
  Western Blot: Sandwich: To detect Eotaxin-2 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 - 1.0 μg/ml is required. In conjunction with Polyclonal Anti-Human Eotaxin-2 as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant Eotaxin-2.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Highly pure (> 98 %) recombinant human Eotaxin-2
• Specificity: This antibody detects Eotaxin-2.
• Formulation: PBS, pH 7.2
  Label: Biotin
  State: Sterile filtered lyophilized Ig fraction
• Reconstitution Method: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/ml.
• Purification: Affinity chromatography
• Conjugation: Biotin
• Storage: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at
  2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C. Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  Entrez Gene 6369 Human
• Background: Eotaxin 2 is a member of the CC chemokine family, based on the presence of the CC motif and homology with other known CC chemokines. Eotaxin 2 cDNA encodes a 119 amino acid residue precursor protein with a 26 amino acid residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N glycosylation site. Mature human eotaxin 2 has a predicted molecular mass of approximately 10.6 kDa. Compared to other CC chemokines, eotaxin 2 exhibits 40 %, 42 %, and 39 % amino acid identity to MCP3, MIP1 alpha, and eotaxin, respectively. Human CC chemokine eotaxin 2 maps to chromosome 7q11.23.
  
  Both eotaxin and eotaxin 2 activate and attract eosinophils and basophils. A receptor for human eotaxin has been identified and found to be the third numbered receptor in the C-C chemokine subfamily of receptors (CCR3. On eosinophils, the effects of eotaxin 2 is inhibited by an CCR3 antibody and cross-desensitized by eotaxin and MCP4, suggesting that all three CC chemokines act through CCR3. Eotaxin 2 mRNA is weakly expressed in activated monocytes and T lymphocytes. Recombinant eotaxin 2 induces chemotaxis of eosinophils, basophils, and resting T lymphocytes but not monocytes and activated T lymphocytes. Eotaxin 2 inhibits colony formation in myleloid progenitor cells.
• Synonyms: C-C motif chemokine 24, Small-inducible cytokine A24, MPIF2, MPIF-2, SCYA24, CK-beta-6