IL21 Rabbit Polyclonal Antibody

CAT#: AP01130PU-N

IL21 rabbit polyclonal antibody, Aff - Purified

Product Images

Sandwich ELISA using Interleukin-21 / IL21 antibody AP01130PU

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  Western Blot unreduced using Interleukin-21 / IL21 antibody AP01130PU

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  Western Blot reduced using Interleukin-21 / IL21 antibody AP01130PU

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Sandwich ELISA: To detect Human IL-21 by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. In conjunction with Biotinylated Anti-Human IL-21 (AP01130BT-N or AP01130BT-S) as a detection antibody, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-21.
  Western Blot: To detect Human IL-21 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-21 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (> 98%) E.coli derived recombinant Human IL-21
• Specificity: This antibody detects Interleukin-21.
• Formulation: PBS, pH 7.2
  State: Aff - Purified
  State: Sterile filtered, lyophilized purified Ig fraction
• Reconstitution Method: Centrifuge vial prior to opening.
  Restore in sterile Water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity chromatography employing immobilized hIL-21 matrix
• Gene Name: Homo sapiens interleukin 21 (IL21), transcript variant 1
• Database Link:
  Entrez Gene 59067 Human
• Background: A novel cytokine related to IL2 and IL15 was recently identified and designated IL21. IL21 has been found to be a powerful growth factor for naive B cells. The receptor for IL21 (IL21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL21 signaling. Both IL21R and the gc are necessary for the IL21 function. IL21 and its receptor activate JAKSTAT signaling pathway. IL21R is expressed in spleen, thymus, natural killer (NK), T and B cell lines. IL21 plays a role in the proliferation and maturation of NK, B and T cell populations.
• Synonyms: IL-21, Za11

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway