CCN2 Rabbit Polyclonal Antibody

CAT#: AP01150PU-N

CCN2 rabbit polyclonal antibody, Aff - Purified

Product Images

Sandwich ELISA using AP01150PU-N CTGF Antibody (Cat.-No AP01150PU)

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  Formalin-Fixed, Paraffin-Embedded Sections of Human breast invasive malignant carcinoma stained with CTGF Antbody (Cat.-No AP01150PU). <em>Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.</em>

Specifications

Product Data

• Applications: ELISA, FN, IHC, WB
• Recommended Dilution: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human CTGF (5.0 µg/ml) a concentration of 6.0-9.0 µg/ml of this antibody is required.
  Sandwich ELISA: To detect Human CTGF (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This affinity purified antibody, in conjunction with Biotinylated antibody Human CTGF (Cat.-No AP01150BT) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human CTGF.
  Western blot: To detect Human CTGF this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
  Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 0.125-0.250 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) E.coli derived recombinant Human CTGF (Cat.-No PA527)
• Specificity: This antibody detects Human Connective Tissue Growth Factor (CTGF).
  Other species not tested.
• Formulation: PBS, pH 7.2 without preservatives.
  State: Aff - Purified
  State: Lyophilized (sterile filtered) purified Ig fraction.
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Immunoaffinity Chromatography employing immobilized Human CTGF matrix.
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Database Link:
  Entrez Gene 1490 Human
• Background: CTGF (connective tissue growth factor) is a 38kDa, cysteine-rich, secreted peptide. It is a new member of the peptide family that include serum-induced immediate early gene products, a v-src-induced peptide and a putative proto-oncogene. Among the many functions of the CTGF gene family are embryogenesis, wound healing and regulation of extracellular matrix production.
• Synonyms: CCN2, HCS24, IGFBP8, Connective tissue growth factor, IGF-binding protein 8,IBP8
• Note: Centrifuge vial prior to opening.