FZD5/FZD8 Rabbit Polyclonal Antibody

CAT#: AP01257PU-N

FZD5/FZD8 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis with extracts from Jurkat cells using FZD5/FZD8 antibody (Cat.-No. AP01257PU-N).

Specifications

Product Data

• Applications: ELISA, IF, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 42-90 of Human Frizzled-5.
• Specificity: This antibody detects endogenous levels of FZD5 / FZD8 protein (region surrounding Trp73).
• Formulation: Phosphate buffered saline (PBS)
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% Sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen
• Predicted Protein Size: ~ 75 kDa
• Background: The frizzled gene, originally identified in Drosophila melanogaster, is involved in the development of tissue polarity. The mammalian homolog of frizzled, as well as several secreted mammalian frizzled-related proteins: FRP-1 (also designated SARP2), FRP-2 (also designated SARP1), FRP-3, FRP-4 and SARP3 (also designated FRP-5), have been identified. The frizzled proteins contain seven transmembrane domains, a cysteine-rich domain in the extracellular region and a carboxy terminal Ser/Thr-xxx-Val motif. They function as receptors for Wnt. The frizzled-1 gene maps to human chromosome 7q21 and is expressed in adult heart, placenta, lung, kidney, pancreas, prostate and ovary and in fetal lung and kidney. Frizzled-2 is expressed in adult heart and fetal brain, lung and kidney. The frizzled related proteins FRP-1, FRP-2, FRP-3, FRP-4 and SARP3 are secreted proteins of approximately 30 kDa that contain regions of homology to the cysteine-rich ligand-binding domain of frizzled and a conserved hydrophilic carboxy terminal.
• Synonyms: Frizzled-5, Frizzled-8