Hormone sensitive lipase (LIPE) Rabbit Polyclonal Antibody

CAT#: AP01276PU-N

Hormone sensitive lipase (LIPE) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of HSL antibody in extracts from Jurkat cells.

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  Immunohistochemistry (IHC) analyzes of HSL antibody in paraffin-embedded human tonsil tissue.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 520-570 of Human HSL.
• Specificity: This antibody detects endogenous levels of HSL protein. (region surrounding Ala546)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ～115.0 kDa
• Gene Name: Homo sapiens lipase E, hormone sensitive type (LIPE)
• Database Link:
  Entrez Gene 16890 MouseEntrez Gene 25330 RatEntrez Gene 3991 Human
• Background: HSL (hormone-sensitive lipase), a cytosolic neutral lipase regulated by reversible phosphorylation, catalyzes the rate limiting step in triglyceride lipolysis. HSL hydrolyzes stored triglycerides to free fatty acids in adipose and heart tissues. In organs with steroidogenic tissues, such as small intestine, HSL converts cholesteryl esters to free cholesterol for steroid hormone production. HSL is highly expressed in jejunal enterocytes and in the mucosa of the small intestine. Two major isoforms of HSL have been described resulting from the use of alternative translational start codons. The short isoform is expressed in adipose tissue while the long isoform is expressed in steroidogenic tissues such as testis. The long isoform, often referred to as testicular HSL contains an N-terminus of approximately 300 amino acids not present in the short isoform of HSL.
• Synonyms: LIPE, Hormone-sensitive lipase, HSL, EC=3.1.1.79

Reference Data

• Protein Pathways: Insulin signaling pathway