JIP3 (MAPK8IP3) Rabbit Polyclonal Antibody

CAT#: AP01286PU-N

JIP3 (MAPK8IP3) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of JIP-3 antibody in extracts from HeLa cells.

• 

  Immunohistochemistry (IHC) analyzes of JIP-3 antibody in paraffin-embedded human lung carcinoma tissue.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 615-664 of Human JIP-3.
• Specificity: This antibody detects endogenous levels of JIP-3 protein. (region surrounding Gln649)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction (>95% pure by SDS-PAGE).
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific Immunogen.
• Predicted Protein Size: ~ 147 kDa
• Database Link:
  Entrez Gene 23162 Human
• Background: c-Jun NH2-terminal kinases (JNKs) are distant members of the MAP kinase family. JNK1 is activated by dual phosphorylation at a Thr-Pro-Tyr motif in response to ultraviolet (UV) light, and it functions to phosphorylate c-Jun at amino terminal serine regulatory sites Ser 63 and Ser 73, resulting in transcriptional activation. Two additional JNK family members, JNK2 and JNK3, have been identified. JIP-1 (for JNK interacting protein-1) has been identified as a cytoplasmic inhibitor of JNK that retains JNK in the cytoplasm, thereby inhibiting JNK-regulated gene expression. Evidence suggests that JNK1 and JNK2 bind to JIP-1 with greater affinity than to ATF-2 and c-Jun, which are targets of the JNK signaling pathway. JIP-1 contains an amino terminal JNK binding domain and a carboxy terminal SH3 domain. ATF-2 and c-Jun also contain the JNK binding domain and are thought to compete with JIP-1 for JNK binding. Multiple splice variants of JIP-1, including JIP-1b, JIP-1c (also designated islet-brain 1 or IB-1), JIP-2a, JIP-2b and JIP-3, have been identified in brain.
• Synonyms: JNK-interacting protein 3, MAPK8IP3, JIP3