CXCR4 Rabbit Polyclonal Antibody

CAT#: AP01296PU-N

CXCR4 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of CXCR-4 antibody at 1/500 dilution Lane 1: Hela whole cell lysate Lane 2: Raw264.7 whole cell lysate Lane 3: H9C2 whole cell lysate.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 310-360 of Human CXCR-4.
• Specificity: CXCR-4 (V340) pAb detects endogenous levels of CXCR-4 protein.
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Predicted Protein Size: ~40 kDa
• Gene Name: Homo sapiens chemokine (C-X-C motif) receptor 4 (CXCR4), transcript variant 1
• Database Link:
  Entrez Gene 12767 MouseEntrez Gene 60628 RatEntrez Gene 7852 Human
• Background: The C-X-C or alpha chemokine family is characterized by a pair of cysteine residues separated by a single amino acid and primarily functions as chemoattractants for neutrophils. The C-X-C family includes IL-8, NAP-2, MSGA and stromal cell derived factor-1 or SDF-1. SDF-1 was originally described as a pre-B cell stimulatory factor, but has now been shown to function as a potent chemoattractant for T cells and monocytes but not neutrophils. Receptors for the C-X-C family are G protein coupled, seven pass transmembrane domain proteins which include IL-8RA, IL-8RB and fusin (variously referred to as LESTR or CXCR-4). Fusin is highly homologous to the IL-8 receptors, sharing 37% sequence identity at the amino acid level. The IL-8 receptors bind to IL-8, NAP-2 and MSGA, while fusin binds to its cognate ligand, SDF-1. Fusin has been identified as the major coreceptor for T-tropic HIV-1 and SDF-1 has been shown to inhibit HIV-1 infection.
• Synonyms: CXC-R4, CXCR-4, Fusin, LCR1, FB22, NPYRL, HM89, SDF1 receptor, LESTR

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, GPCR, Transmembrane
• Protein Pathways: Axon guidance, Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Endocytosis, Leukocyte transendothelial migration