HNF 4 alpha (HNF4A) Rabbit Polyclonal Antibody

CAT#: AP01345PU-N

HNF 4 alpha (HNF4A) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of HNF4a antibody in extracts from HT-29 cells.

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  Immunohistochemistry (IHC) analyzes of HNF4 alpha antibody in paraffin-embedded human liver carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) using PBS instead of primary antibody.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 280-330 of Human HNF4α.
• Specificity: This antibody detects endogenous levels of HNF4α protein. (region surrounding Lys298)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~52 kDa
• Gene Name: hepatocyte nuclear factor 4 alpha
• Database Link:
  Entrez Gene 3172 Human
  UniProt ID P41235
• Background: HNF-1 (α and β), HNF-3 (α, β and γ), HNF-4 (α and γ), and HNF-6 compose, in part, a homoeprotein family designated the Hepatocyte Nuclear Factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. HNF-3α, HNF-3β and HNF-3γ regulate the transcription of numerous hepatocyte genes in adult liver. HNF-3α and HNF-3β have also been shown to be involved in gastrulation events such as body axis formation. HNF-4α and HNF-4γ have been shown to be important for early embryo development. HNF-4α is expressed in liver, kidney, pancreas, small intestine, testis and colon; and HNF-4γ is expressed in each of these tissues except liver. HNF-6 has been shown to bind to the promoter of HNF-3β, which indicates a potential role of HNF-6 in gut endoderm epithelial cell differentiation. Evidence suggests that HNF-6 may also be a transriptional activator for at least 22 other hepatocyte-enriched genes, including cytochrome P450 2C13 and α-1 antitrypsin.
• Synonyms: HNF4A, HNF4, NR2A1, Hepatocyte nuclear factor 4-alpha, Transcription factor HNF-4, Transcription factor 14