PI 3 Kinase p85 alpha (PIK3R1) (alpha/gamma) Rabbit Polyclonal Antibody

CAT#: AP01368PU-N

PI 3 Kinase p85 alpha (PIK3R1) (alpha/gamma) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of PI 3-kinase p85a/? antibody (Cat.-No.: AP01368PU-N) in extracts from NIH/3T3 cells.

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  Immunohistochemistry (IHC) analyzes of PI 3-kinase p85a/? antibody (Cat.-No.: AP01368PU-N) in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of PI 3-kinase p85 alpha + gamma protein.
• Formulation: Phosphate buffered saline (PBS) with 15 mM Sodium Azide as preservative, approx. pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 86 kDa
• Gene Name: Homo sapiens phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1), transcript variant 2
• Database Link:
  Entrez Gene 18708 MouseEntrez Gene 25513 RatEntrez Gene 5295 Human
• Background: The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
• Synonyms: PIK3R1, GRB1, PI3K, PIK3R3, p55PIK

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Acute myeloid leukemia, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Insulin signaling pathway, Jak-STAT signaling pathway, Leukocyte transendothelial migration, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Phosphatidylinositol signaling system, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus, VEGF signaling pathway