CYP7B1 Rabbit Polyclonal Antibody

CAT#: AP01374PU-N

CYP7B1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of CYP7B1 antibody (Cat.-No.: AP01374PU-N) in extracts from LOVO cells.

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  Immunohistochemistry (IHC) analyzes of CYP7B1 antibody (Cat.-No.: AP01374PU-N) in paraffin-embedded human skeletal muscle tissue

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of CYP7B1 protein. (region surrounding Gln127)
• Formulation: PBS pH~7.2 with 0.05% Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction (>95% pure by SDS-PAGE)
• Concentration: 1.0 mg/ml
• Purification: Affinity-Chromatography using epitope-specific immunogen
• Predicted Protein Size: ~ 58 kDa
• Gene Name: Homo sapiens cytochrome P450 family 7 subfamily B member 1 (CYP7B1), transcript variant 1
• Database Link:
  Entrez Gene 9420 Human
• Background: P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds including cholesterol. CYP8B1 moderates the ratio of cholic acid over chenodeoxycholic acid to control the solubility of cholesterol.
  P450 cholesterol 7-hydroxylase, CYP7A1, is the rate limiting enzyme of bile acid synthesis in the liver, and its expression is mediated by the bile acid receptor FXR. CYP27A1 catalyzes vitamin D3 25-hydroxylation and is localized to the mitochondria in kidney and liver. CYP7B1 (oxysterol 7-α-hydroxylase) functions as an enzyme in the alternate bile acid synthesis pathway. Specifically, CYP7B1 metabolizes 25-and 27-hydroxycholesterol. The gene encoding human CYP7B1 maps to chromosome 8q21.3. Mutations in the CYP7B1 gene may cause a metabolic defect in bile acid synthesis characterized by elevated urinary bile acid excretion, severe cholestasis, cirrhosis and liver synthetic failure.
• Synonyms: Cytochrome P450 7B1

Reference Data

• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Primary bile acid biosynthesis