PAK4 Rabbit Polyclonal Antibody

CAT#: AP01447PU-N

PAK4 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of PAK4/5/6 antibody (Cat.-No.: AP01447PU-N) in extracts from K562 cell treated with PMA 125ng/ml 30'.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: PAK4/5/6 antibody detects endogenous levels of PAK4/5/6 protein. (region surrounding Val469)
• Formulation: Phosphate buffered saline (PBS) with 0.05% Sodium Azide as preservative, approx. pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Predicted Protein Size: ~ 80 kDa
• Gene Name: Homo sapiens p21 (RAC1) activated kinase 4 (PAK4), transcript variant 2
• Database Link:
  Entrez Gene 70584 MouseEntrez Gene 292756 RatEntrez Gene 10298 Human
• Background: Three recently identified isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiaekinase STE20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is MEK kinase, an upstream effector of MEK4 which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP-dependent manner with Rac1 and Cdc42, they do not interact with Rho.
• Synonyms: PAK 4, PAK-4, p21-activated kinase 4, KIAA1142, p21-activated kinase 7, PAK-7, p21-activated kinase 5, PAK-5, KIAA1264 , p21-activated kinase 6, PAK-6

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, ErbB signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway