NCF1 Rabbit Polyclonal Antibody

CAT#: AP01461PU-N

NCF1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of p47-phox antibody (Cat.-No.: AP01461PU-N) in extracts from MCF7 (Lane 1) and HeLa (Lane 2) cells at 1/500 dilution.

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  Immunohistochemistry (IHC) analyzes of p47-phox antibody (Cat.-No.: AP01461PU-N) in paraffin-embedded human testis tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of p47-phox protein. (region surrounding Pro366)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 15 mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Predicted Protein Size: ~ 45 kDa
• Gene Name: Homo sapiens neutrophil cytosolic factor 1 (NCF1)
• Database Link:
  Entrez Gene 17969 MouseEntrez Gene 114553 RatEntrez Gene 653361 Human
• Background: The heredity disease chronic granulomatous disease (CGF) has been linked to mutations in p47-phox and p67-phox. The cytosolic proteins p47-phox and p67-phox, also designated neutrophil cytosol factor (NCF)1 and NCF2, respectively, are required for activation of the superoxide-producing NADPH oxidase in neutrophils and other phagocytic cells. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex. Both p47-phox and p67-phox contain two Src homology 3 (SH3) domains. The C-terminal SH3 doamin of p67-phox has been shown to interact with the proline rich domain of p47-phox, suggesting that p47-phox may faciliate the transport of p67-phox to the membrane.
• Synonyms: NCF-1, Neutrophil cytosol factor 1, NOXO2, Nox organizer 2, SH3PXD1A

Reference Data

• Protein Pathways: Chemokine signaling pathway, Fc gamma R-mediated phagocytosis, Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton