p21 (CDKN1A) pThr145 Rabbit Polyclonal Antibody

CAT#: AP01654PU-N

p21 (CDKN1A) pThr145 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of p21 Cip1 pThr145 antibody in extracts from HeLa cells.

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  Immunohistochemistry (IHC) analysis of p21Cip1 pThr145 antibody in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/10000-1/20000.
  Western Blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of p21Cip1 p53 protein when phosphorylated at Thr145
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: ~21.0 kDa
• Gene Name: cyclin-dependent kinase inhibitor 1A
• Database Link:
  Entrez Gene 1026 Human
  UniProt ID P38936
• Background: The p53-inducible gene product, p21(WAF1/CIP1), plays a critical role in regulating the rate of tumor incidence. p21 is a kinase inhibitor that was originally identified as a cyclin-dependent kinase and PCNA-binding protein ble to inhibit Cdk catalytic activity and as a gene whose expression was induced by the tumor suppressor protein p53. p21 contributes to the regulation of cell division, mediation of negative growth signals, differentiation and senescence, modulation of the apoptotic response and activaion of certain cyclin-Cdks in response to mitogenic signals. Serine 146 is a phosphorylation site within the carboxy terminal regulatory domain of p21 and when modified in vivo influences p21-PCNA interactions.
• Synonyms: CAP20, CDKN1, CIP1, MDA6, MDA-6, PIC1, SDI1, WAF1