Glypican 4 (GPC4) (509-520) Rabbit Polyclonal Antibody

CAT#: AP02029SU-N

Glypican 4 (GPC4) (509-520) rabbit polyclonal antibody, Serum

Product Images

Western Blot analysis of Glypican-4 expression in tissues and cell lines. Proteoglycans from the cell or tissue extracts were separated by SDS-PAGE either prior to (N) or after (H) digestion with heparinase/heparitinase. Immunoblotting with Glypican-4 Antibody Cat.-No AP02029SU detects native Glypican-4 in the range of 90-200 kDa, the size of the core was Approx. 57 kDa. TF-1, Human haematopoietic-progenitor cell line; BM, bone marrow; Mr Molecular mass. Siebertz B et al. (1999) Biochem. J. 344: 937-43.

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  Immunohistochemistry of Glypica-4 staining in section of Human Kidney tissue. The section was incubated with Glypican-4 Antibody Cat.-No AP02029SU and detected using a peroxidase-conjugated secondary antibody according to standard procedures. In the cortex AP02029SU stains weakly the glomeruli, while the luminal surface of epithelial cells in the proximal tubules stained strongly. Original magnification: x1280. Siebertz B et al. (1999) Biochem. J. 344: 937-43.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: Western blot: 1/1000 (Ref.1).
  Flow Cytometry (Ref.1).
  Immunohistochemistry on Paraffin Sections: 1/1000 (Ref.1).
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic Glypican-4 (aa 509-520) coupled to KLH.
• Specificity: Specific for a core protein epitope (aa 509-520) of Glypican-4.
• Formulation: State: Serum
  State: Lyophilized Serum
• Reconstitution Method: Restore in aqua bidest to initial volume.
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 2239 Human
• Background: Glypican-4, previously known as K-Glypican, is a GPI-linked cell surface heparan sulfate proteoglycan of the glypican gene family, that comprises 6 members at present. Its function is only partially understood at present. However, glypican-4 seems to be involved in the control of cell growth and diffe-rentiation, especially in the development of the central nervous system and the kidney. Furthermore, Glypican-4 is expressed by hematopoetic stem cells and bone marrow stromal cells.
• Synonyms: Glypican 4, K-glypican