PLAT Goat Polyclonal Antibody

CAT#: AP02244SU-S

PLAT goat polyclonal antibody, Serum

Specifications

Product Data

• Applications: ELISA, IHC
• Recommended Dilution: ELISA.
  Immunohistochemstry on Frozen Sections: 1/50-1/100.
  Immunohistochemstry on Paraffin Sections: 1/50 (Pretreatment with 0.05% Pronase E for 20 minutes).
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Purified Human tissue Plasminogen Activator [tPA]
• Specificity: This antibody detects free tissue Plasminogen Activator (tPA) and tPA inhibitor complexes.
  There were no cross reactivities obtained with Human uPA and PAI-2.
• Formulation: State: Serum
  State: Lyophilized Serum
• Reconstitution Method: Restore in aqua bidest to initial volume.
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 5327 Human
• Background: TPA converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. TPA binds to fibrin with high affinity; this leads to an increase in the catalytic efficiency of the enzyme of 100- to 1000-fold, due to an increase in affinity for plasminogen. Similarly, binding to heparin increases the activation of plasminogen. Binding to laminin and fibronectin has also been demonstrated. TPA also binds to mannose receptor and the low density lipoprotein receptor related protein (LRP1). These proteins are involved in TPA clearance. TPA binds to annexin II and to cytokeratin 8. As yet unidentified interactions on endothelial cells and vascular smooth muscle cells (VSMC) lead to a 100-fold stimulation of plasminogen activation. Binding to VSMC reduces TPA inhibition by PAI-1 by 30-fold
• Synonyms: tPA, Tissue-type plasminogen activator, Alteplase, Reteplase
• Note: Reference no.: LocusID 5327