Collagen XVII (COL17A1) (507-548) Rabbit Polyclonal Antibody

CAT#: AP02251PU-S

Collagen XVII (COL17A1) (507-548) rabbit polyclonal antibody, Ig Fraction

Product Images

Immunocytochemistry of Collagen XVII staining in cultured Human skin squamous carcinoma DJM-1 cells. The cells were grown on glass slides, fixed in 4% paraformaldehyde and incubated with AP02251PU. AP02251PU stains Collagen XVII at hemidesmosomes. Scale bar = 10 µm. Hirako Y et al. (2003), J. Biochem 133 (2): 197-206.

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA: 1/5000.
  Western blot: 1/500 (Ref.2)
  Immunocytochemistry (Ref.3).
  Immunocytochemistry on Frozen Sections: 1/100 (Ref.3).
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Glutathione-S-transferase fusion protein containing the sequence of the immunodominant region of human type XVII collagen / BP180 (aa 507-548)
• Specificity: This antibody detects Human type XVII Collagen / BP180 (aa 507-548).
• Formulation: 50mM Tris, pH 7.4
  State: Ig Fraction
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore in aqua bidest to a concentration of 1 mg/ml.
• Purification: Protein G Chromatography
• Storage: Store lyophilized at 2-8°C and reconstituted at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: Homo sapiens collagen type XVII alpha 1 chain (COL17A1)
• Database Link:
  Entrez Gene 1308 Human
• Background: Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in the gene coding for collagen XVII are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD 1, is generated by proteolytic processing of the full length form. Two transcript variants, one resulting from alternative splicing in the 3' UTR, have been identified for this gene.
• Synonyms: COL17A1, BP180, BPAG2, Bullous pemphigoid antigen 2, LAD-1, LABD97

Reference Data

• Protein Families: Transmembrane