EGF Rabbit Polyclonal Antibody

CAT#: AP02293SU-N

EGF rabbit polyclonal antibody, Serum

Product Images

Western Blot analysis of AP02293SU specificity: Crude extract from S. domuncula tissue was separated by SDS-PAGE and immunoblotted with AP02293SU (1/500). Lane a: Crude extract stained with Coomassie Brilliant Blue. Lane b: AP02293SU detects a 5 kDa band which corresponds to a processed form of EGF from the 78 kDa precursor. Lane d: AP02293SU preabsorbed with recombinant EGF precursor. Perovic-Ottstadt S et al. Eur J Biochem. 271:1924-1937.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/50000.
  Western blot: 1/500 (Ref.1).
  Immunohistochemistry: 1/50.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant EGF precursor from Suberites domuncula.
• Specificity: This antibody detects EGF precursor domain number 1, 2 and 3.
• Formulation: State: Serum
  State: Lyophilized serum
• Reconstitution Method: Restore in aqua bidest to initial volume.
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens epidermal growth factor (EGF), transcript variant 1
• Database Link:
  Entrez Gene 1950 Human
• Synonyms: beta-urogastrone; HOMG4; URG

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transmembrane
• Protein Pathways: Bladder cancer, Cytokine-cytokine receptor interaction, Endocytosis, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton