MAPT / TAU pSer396 Rabbit Polyclonal Antibody

CAT#: AP02394PU-N

MAPT / TAU pSer396 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extract from mouse brain tissue using Tau antibody pSer396 (AP02394PU-N or AP02394PU-S).

• 

  Immunohistochemical analysis of Paraffin-Embedded Rat Hippocampal region tissue from model with Alzheimer's Disease using Tau antibody pSer396 (AP02394PU-N or AP02394PU-S)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500~1/1000.
  Immunohistochemistry: 1/50~1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of Serine 396 (Y-K-S(p)-P-V) derived from Human Tau.
  Peptide-KLH.
• Specificity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  This phospho antibody detects endogenous levels of Tau only when phosphorylated at serine396.
• Formulation: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: 1.0 mg/ml
• Purification: Immunoaffinity Chromatography.
• Predicted Protein Size: 48, 62, 78 kda (Predicted)
• Background: Tau is a neuronal microtubule associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerisation and stabilise microtubules, but it also serves to link certain signalling pathways to the cytoskeleton. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF) and neurofibrillary lesions in Alzheimer's disease (AD) brain. Hyperphosphorylation impairs the microtubule binding function of Tau, resulting in the destabilisation of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Hyperphosphorylated tau is also found in a range of other central nervous system disorders. Numerous serine/threonine kinases, including GSK3 beta, PKA, Cdk5, and casein kinase II can phosphorylate Tau.
• Synonyms: MAPTL, MTBT1, Microtubule-associated protein tau, PHF-tau, Neurofibrillary tangle protein, Paired helical filament-tau