MAPT / TAU pSer262 Rabbit Polyclonal Antibody

CAT#: AP02403PU-N

MAPT / TAU pSer262 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 2. Western blot analysis of extracts from mouse brain tissue using Tau antibody (AP02633PU, Lane 1 and 2) and Tau (phospho-Ser262) antibody (AP02403PU, Lane 3 and 4).

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  Figure 1. Immunohistochemical analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer's Disease using Tau (phospho-Ser262) antibody (AP02403PU).

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  Figure 3. Immunofluorescence staining of methanol-fixed HeLa cells using Tau (phospho-Ser262) antibody (#AP02403PU, Red).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunofluorescence: 1/100-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of serine 262 (I-G-S(p)-T-E) derived from Human Tau.
• Specificity: AP02403PU-N Antibody detects endogenous levels of Tau only when phosphorylated at Serine 262.
• Formulation: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific phosphopeptide.
  Non-phosphospecific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Predicted Protein Size: 48, 62, 78 kd.
• Background: Tau is a neuronal microtubule associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerisation and stabilise microtubules, but it also serves to link certain signalling pathways to the cytoskeleton. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF) and neurofibrillary lesions in Alzheimer's disease (AD) brain. Hyperphosphorylation impairs the microtubule binding function of Tau, resulting in the destabilisation of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Hyperphosphorylated tau is also found in a range of other central nervous system disorders. Numerous serine/threonine kinases, including GSK3 beta, PKA, Cdk5, and casein kinase II can phosphorylate Tau.
• Synonyms: MAPTL, MTBT1, Microtubule-associated protein tau, PHF-tau, Neurofibrillary tangle protein, Paired helical filament-tau