MAPT / TAU pSer404 Rabbit Polyclonal Antibody

CAT#: AP02404PU-N

MAPT / TAU pSer404 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extract from Mouse brain tissue using Tau antibody (phospho-Ser404) Cat.-No. AP02404PU and the same antibody preincubated with blocking peptide.

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  Immunohistochemical analysis of paraffin-embedded Rat hippocampal region tissue from a model with Alzheimer's Disease using Tau antibody (phospho-Ser404) Cat.-No. AP02404PU.

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  Immunofluorescence staining of methanol-fixed HeLa cells using Tau antibody (phospho-Ser404) Cat.-No. AP02404PU.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Incubate Membrane with diluted antibody in 5% nonfat milk, 1xTBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunofluorescence: 1/100-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide around phosphorylation site of Serine 404 (D-T-Sp-P-R) derived from Human Tau.
• Specificity: AP02404PU antibody detects endogenous levels of Tau only when phosphorylated at Serine 404.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 50% Glycerol
  Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Predicted Protein Size: 48, 62, 78 KDa
• Background: Tau is a neuronal microtubule associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerisation and stabilise microtubules, but it also serves to link certain signalling pathways to the cytoskeleton. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF) and neurofibrillary lesions in Alzheimer's disease (AD) brain. Hyperphosphorylation impairs the microtubule binding function of Tau, resulting in the destabilisation of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Hyperphosphorylated tau is also found in a range of other central nervous system disorders. Numerous serine/threonine kinases, including GSK3 beta, PKA, Cdk5, and casein kinase II can phosphorylate Tau.
• Synonyms: MAPTL, MTBT1, Microtubule-associated protein tau, PHF-tau, Neurofibrillary tangle protein, Paired helical filament-tau