PAK1 pThr423/402/421 Rabbit Polyclonal Antibody

CAT#: AP02447PU-S

PAK1 pThr423/402/421 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PAK1/PAK2/PAK3 (Phospho-Thr423/Thr402/Thr421) antibody.

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  Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using PAK1/PAK2/PAK3 (Phospho- Thr423/Thr402/Thr421) antibody (Red).

Specifications

Product Data

• Applications: IF, IHC
• Recommended Dilution: Immunofluorescence: 1/100-1/200.
  Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human PAK1 around the phosphorylation site of threonine 423 (R-S-TP-M-V).
• Specificity: This antibody detects endogenous levels of PAK1/PAK2/PAK3 only when phosphorylated at Threonine 423/402/421.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens p21 protein (Cdc42/Rac)-activated kinase 1 (PAK1), transcript variant 1
• Database Link:
  Entrez Gene 18479 MouseEntrez Gene 29431 RatEntrez Gene 5058 Human
• Synonyms: PAK 1, PAK-1, Alpha-PAK, PAK alpha, p21-activated kinase 1, p65-PAK, PAK 2, PAK-2, Gamma-PAK, PAK gamma, PAK65, p21-activated kinase 2, p58, PAK 3, PAK-3, Beta-PAK, PAK beta, p21-activated kinase 3, Oligophrenin-3, OPHN3

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Axon guidance, Chemokine signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway