MEK4 (MAP2K4) pSer80 Rabbit Polyclonal Antibody

CAT#: AP02454PU-S

MEK4 (MAP2K4) pSer80 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 2. Western blot analysis of extract from 293 cells treated or untreated with UV, using SEK1/ MKK4 (phospho-Ser80) antibody.

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  Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using SEK1/MKK4 (phospho-Ser80) antibody.

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  Figure 3. Immunofluorescence staining of methanol-fixed HeLa cells using SEK1/MKK4 (phospho-Ser80) antibody (Red).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunofluorescence: 1/100-1/200.
  Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human SEK1/MKK4 around the phosphorylation site of serine 80 (T-H-Sp-I-E).
• Specificity: SEK1/MKK4 (phospho-Ser80) antibody detects endogenous levels of SEK1/MKK4 only when phosphorylated at Serine 80.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  Entrez Gene 6416 Human
• Background: MEK4 is a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8 / JNK1, MAPK9 / JNK2, and MAPK14 / p38, but not MAPK1 / ERK2 or MAPK3 / ERK3. This kinase is phosphorylated, and thus activated by MAP3K1 / MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.
• Synonyms: JNK-activating kinase 1, MAP Kinase Kinase 4, SAPK/ERK kinase 1, PRKMK4, SERK1, MEK-4, MAPKK4, MKK4