RPS6 pSer235 Rabbit Polyclonal Antibody

CAT#: AP02490PU-S

RPS6 pSer235 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Western blot analysis of the extracts from 293 cells untreated or treated with serum (10%, 15min), using Ribosomal protein antibody (Lane 1 and 2) and S6 Ribosomal protein (pSer235) antibody (Line 3 and 4).

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  Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using S6 Ribosomal protein (phospho-Ser235) antibody (Red).

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunofluorescence: 1/100-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human S6 Ribosomal protein around the phosphorylation site of serine 235 (R-L-SP-S-L).
• Specificity: This antibody detects endogenous levels of S6 Ribosomal protein only when phosphorylated at Serine 235.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: Homo sapiens ribosomal protein S6 (RPS6)
• Database Link:
  Entrez Gene 20104 MouseEntrez Gene 29304 RatEntrez Gene 6194 Human
• Synonyms: Phosphoprotein NP33, 40S ribosomal protein S6

Reference Data

• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway, Ribosome