ERK1 (MAPK3) pThr202 Rabbit Polyclonal Antibody

CAT#: AP02498PU-N

ERK1 (MAPK3) pThr202 rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot analysis of extracts from 293 cells untreated or treated with PMA for the indicated times, using p44/42 MAP Kinase antibody (pThr202)

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  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase antibody (pThr202) (left) or the same antibody preincubated with Blocking peptide (right).

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  Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic, nuclear staining using p44/42 MAP Kinase antibody (pThr202)

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000. Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunofluorescence: 1/100-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Syntetic phosphopeptide derived from Human p44/42 MAP Kinase around the phosphorylation site of Threonine 202 (F-L-Tp-E-Y).
• Specificity: This antibody detects endogenous levels of p44/42 MAP Kinase only when phosphorylated at Threonine 202.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl and 50% Glycerol
  State: Aff - Purified
  State: Liquid purified IgG fraction
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 42, 44 kDa
• Database Link:
  Entrez Gene 5595 Human
• Background: Both p44 and p42 MAP kinases (Erk1 and Erk2) function in a protein kinase cascade that plays a critical role in the regulation of cell growth and differentiation. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1] or 183 and 185 of rat Erk2) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). Both kinases are known to weakly autophosphorylate on tyrosine.
• Synonyms: ERK-1/ERK-2, MAPK1/MAPK2, P42/P44-MAPK