S6K1 (RPS6KB1) pSer411 Rabbit Polyclonal Antibody

CAT#: AP02516PU-N

S6K1 (RPS6KB1) pSer411 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 2. Western blot analysis of extracts from 293 cells, untreated or treated with insulin (10u/ml, 15min), using p70 S6 Kinase antibody (Lane 1 and 2) and p70 S6 Kinase pSer411 antibody (Lane 3 and 4).

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  Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using p70 S6 Kinase pSer411 antibody

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  Figure 3. Immunofluorescence staining of methanol-fixed MCF7 cells using p70 S6 Kinase pSer411 antibody (Red).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunofluorescence: 1/100-1/200.
  Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human p70 S6 Kinase around the phosphorylation site of serine 411 (I-R-SP-P-R).
• Specificity: This antibody detects endogenous levels of p70 S6 Kinase only when phosphorylated
  at Serine 411.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: Homo sapiens ribosomal protein S6 kinase B1 (RPS6KB1), transcript variant 1
• Database Link:
  Entrez Gene 72508 MouseEntrez Gene 83840 RatEntrez Gene 6198 Human
• Synonyms: Ribosomal protein S6 kinase I, S6K1, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA, Serine/threonine-protein kinase 14A

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, ErbB signaling pathway, Fc gamma R-mediated phagocytosis, Insulin signaling pathway, mTOR signaling pathway, TGF-beta signaling pathway