EGFR Rabbit Polyclonal Antibody

CAT#: AP02613PU-S

EGFR rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot analysis of extracts from HUVEC and MDA cells using EGFR antibody

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  Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using EGFR antibody (left) or the same antibody preincubated with blocking peptide (right)

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  Immunofluorescence staining of Methanol-Fixed MCF cells using EGFR antibody

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000. Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunofluorescence: 1/100-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around amino acids 1068~1072 (R-Y-S-S-D) derived from Human EGFR.
• Specificity: This antibody detects endogenous levels of total EGFR protein.
• Formulation: PBS (without Mg²⁺ and Ca²⁺), pH 7.4 containing 150mM NaCl and 50% Glycerol
  State: Aff - Purified
  State: Liquid purified IgG fraction
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 175 kDa
• Database Link:
  Entrez Gene 1956 Human
• Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
• Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1