Phosphoserine Rabbit Polyclonal Antibody

CAT#: AP03033HR-N

Phosphoserine rabbit polyclonal antibody, HRP, Aff - Purified

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA: 1/1000.
  Western blot: 1/500.
  2 μg/mL of this antibody was sufficient for detection of phosphorylation signal in western blot analysis using Human MMRU cells treated with 0.1 μM Okadaic Acid.
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Phosphoserine conjugated to KLH
• Specificity: This antibody recognizes proteins phosphorylated on Serine residues.
  Does not cross-react with Phosphotyrosine.
• Formulation: PBS, pH 7.0
  Label: HRP
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.1% BSA, 50% Glycerol
  Preservative: 0.01% Proclin150
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: HRP
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Background: Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1).
  Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).