Acetylated Lysine Rabbit Polyclonal Antibody

CAT#: AP03035HR-N

Acetylated Lysine rabbit polyclonal antibody, HRP, Aff - Purified


USD 490.00

5 Days*

Size
    • 400 ul

Product Images

Specifications

Product Data
Applications ELISA, IF, IP, WB
Recommended Dilution ELISA. 
Western blot: A 1/250 dilution of this HRP- conjugated antibody AP03035HR-N was sufficient to detect the acetylated histone from TSA treated mouse spleen cell in western blot analysis (Ref.6). 
Immunofluorescence.
Immunoprecipitation (Ref.7).
Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Acetylated KLH
Specificity This antibody detects proteins containing Acetylated Lysine residues in SDS-PAGE immunoblots (Multi-species).
Formulation PBS
Label: HRP
State: Aff - Purified
State: Liquid purified Ig fraction
Stabilizer: 50% Glycerol
Preservative: 0.09% Sodium Azide
Concentration lot specific
Purification Affinity Chromatography
Conjugation HRP
Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.

Stability Shelf life: one year from despatch.

Background Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1).
The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2).
Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3).
The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anticancer drugs currently in development (5).
Synonyms AcK, acetyl Lysine, acetyl-Lysine
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.