p53 (TP53) Sheep Polyclonal Antibody

CAT#: AP05006PU-N

p53 (TP53) sheep polyclonal antibody, Purified

Specifications

Product Data

• Applications: IHC, IP, WB
• Recommended Dilution: WB: 1-10 µg/ml.
  Immunoprecipitation.
  Immunohistochemistry on formalin-fixed and paraffin embedded tissue.
• Reactivities: Human, Mouse, Rat
• Host: Sheep
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: GST p53 fusion protein containing amino acids 1-393 of human p53.
• Specificity: This antibody reacts to GST p53.
• Formulation: Phosphate buffered saline with 0.08% sodium azide
  State: Purified
  State: Liquid purified Ig (0.2µm sterile filtered)
• Concentration: lot specific
• Purification: Ammonium Sulfate Precipitation
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 7157 Human
• Background: GST p53 fusion protein containing amino acids 1-393 of human p53was used as immunogen. p53 has a molecular weight of 53kDa p53 is a transcription factor that can inhibit cell cycle progression or induce apoptosis in response to stress or DNA damage. Disruption of the p53 signalling pathway through various mechanisms is the most common alteration in human cancer occuring in over half of all tumors. The p53 protein is short lived and expressed at low levels in normal cells but accumulates and/or is activated in cells that have undergone genotoxic damage or oncogene activation. Many tumor derived and transformed cell lines express elevated levels of mutant p53 protein.
• Synonyms: Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13