ADAM10 Rabbit Polyclonal Antibody

CAT#: AP05089PU-N

ADAM10 rabbit polyclonal antibody, Purified

Product Images

Western blot analysis: ADAM10 antibody staining of Jurkat cell lysate at 0.5 ug/ml. Lane 1: Without Blocking peptide Lane 2: With Blocking peptide

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western Blot: 0.25-1 µg/ml.
  Detects an 85 kDa band can be detected in Jurkat cell lysates which may represent precursor protein. A faint 60 kDa band is also detected in some cell lines, including Jurkat, which may be processed mature protein.
  Positive Control/Tissue Expression: Jurkat whole cell lysate.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to amino acids 732-748 off the Human ADAM10.
• Specificity: This antibody recognizes ADAM10.
• Formulation: Phosphate buffered saline solution containing 0.02% Sodium Azide as a preservative.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Storage: The antibody can be shipped at ambient temperature.
  Store (in aliquots) at -20°C.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 102 Human
• Background: Proinflammatory cytokine tumor necrosis factor-a (TNF-a) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-a, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor.
  ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-a, notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively, (1,2,4). ADAM10 mRNA is expressed in a variety of human and bovine tissues (1,4).
• Synonyms: KUZ, MADM, Kuzbanian protein homolog