BNIP3L Rabbit Polyclonal Antibody

CAT#: AP05094PU-N

BNIP3L rabbit polyclonal antibody, Purified

Product Images

Western blot analysis using anti-Bnip3L (IN) antibody at 1 ug/ml on K562 whole cell lysate in the absense (1) and presense (2) of immunogenic peptide.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western Blot: 0.5-1 µg/ml. Detects a 40 kDa band in K562 cell lysate which represents the monomer of Bnip3L.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to amino acids 77 to 92 of the Human Bnip3L protein.
  Immunogen sequence is identical in the mouse protein.
• Specificity: This antibody recognizes Bnip3L (IN) (nix).
• Formulation: PBS containing 0.02% Sodium Azide as a preservative
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Antigen Immunoaffiinity Chromatography
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 665 Human
• Background: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bnip3L, Bnip3a, and Nix (for Nip3-like protein X) (1-3). Bnip3L/Bnip3a/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Overexpression of Bnip3L induces apoptosis (2,3). Bnip3L interacts with and overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3L is localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues (1,2). Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins.
• Synonyms: BNIP3H, NIX