Farnesyl Rabbit Polyclonal Antibody

CAT#: AP05110PU-N

Farnesyl rabbit polyclonal antibody, Purified

Product Images

Immunofluorescence assay using anti-Farnesyl antibody on plasmids encoding isoprenylated protein and visualized using FITC-conjugated goat anti-rabbit antibody.

Specifications

Product Data

• Applications: ELISA, IF
• Recommended Dilution: ELISA: Detects farnesyl motif derived from isoprenylated C-A-A-X sequence. Also cross-reacts with geranylgeranyl motif. Detects KLH as well.
  Immunofluorescence
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Antibody developed using Farnesyl cysteine conjugated to KLH.
• Specificity: This antibody recognizes Farnesyl.
• Formulation: Phosphate buffered saline with 0.08% Sodium Azide
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: 1.0 mg/ml
• Purification: Ammonium Sulfate Precipitation
• Background: Protein isoprenylation is a post-translational modification that affects about 0.5% of cellular proteins and is essential for the biological activity of proteins. Two enzymes catalyze the attachment of two prenyl groups to the sulfhydryl group of carboxyl-terminal cysteine groups. Proteins which are prenylated by these enzymes have a distinct motif at the C-terminal of the protein, C-A1-A2-X (C = Cysteine, A 1 & A2 = aliphatic amino acids). The two enzymes involved in this transfer are farnesyltransferase and geranylgeranyltransferase. These transfer a 15 carbon farnesyl or a 20 carbon geranygeranyl, respectively, from a prenyl-pyrophosphate to the protein. Examples of proteins containing this C-A-A-X motif are members of the Ras small G protein family, the nuclear lamins and the gamma subunit of trimeric G proteins. Prenylation of proteins is necessary for membrane association of proteins as well as protein-protein interactions and the nature of the linked isoprenyl group can influence the protein interactions, such as the interaction between G proteins and receptors.